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[Study on multipotential differentiation of human bone marrow mesenchymal stem cells in vitro].

Ying C. Chang, Xin Qi, Li-sha Bu, Zhongxin Xu

Abstract

OBJECTIVE: To study the capability of human bone marrow mesenchymal stem cells (MSCs) to differentiate into adipocyte, osteoblasts, and neurocytes under different experimental conditions in vitro. METHODS: The induced human bone marrow MSCs were examined by cytochemistry staining and immunohistochemistry staining. RESULTS: Induced human bone marrow MSCs formed alkaline phosphatase-positive aggregates and Von Kossa stain-positive nodules under the condition of osteogenic differentiation. Under the condition of adipogenic differentiation, the isolated human bone marrow MSCs formed oil red-O-positive cells. Immunohistochemistry results showed that differentiated human bone marrow MSCs expressed neuron-specific enolase (NSE) and neurofilament (NF-M). CONCLUSION: Human bone marrow MSCs have the capability of multipotential differentiation in vitro.

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Key concepts: Mesenchymal stem cell, Bone marrow, Pathology, Alkaline phosphatase, Medicine, Oil Red O, Adipogenesis, Immunohistochemistry