An Experimental Study of Isolation and Pluripotential of Rat Bone Marrow Stem Cells

Objective:To establish an in vitro isolation and culture system of rat bone marrow stem cells and to verify their pluripotential. Methods:Density gradient centrifugation was used to isolate bone marrow MSCs from SD rats and their morphological characteristics were examined under microscope. The MSCs were also undergone osteogenic, neurogenic and myogenic induction and cytochemical and immunocytochemical staining were performed to verify their multipotential. Results:High purity of bone marrow MSCs was successfully obtained and they showed good proliferation ability. Both ALP activity and mineraliztion node staining were positive in MSCs after osteogenic differentiation and MSCs specially expressed neuron markers, NSE and NF200, and myoblast markers, desmin and connexin-43, after neurogenic and myogenic differentiation, respectively. Conclusion:The isolation and culture system of rat bone marrow MSCs was successfully established and the MSCs sustained their osteogenic, neurogenic and myogenic differentiation potential in vitro.