2015•Journal of Shihezi UniversityRequires access
Isolation and Culture of Mouse Mesenchymal Stem Cells by Using the Whole bone Marrow Adherence Method
Zhang Men
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Abstract
To establish a stable and efficient method for isolation and culture of mouse bone marrow mesenchymal stem cells(MSCs). MSCs were isolated by adherence to plastic plate from C57BL/6 mice bone marrow. The surface markers of the third generation were identified by flow cytometry. Osteogenic and chondrogenic inductions were performed in vitro to assess the differentiation capacity. As the result showed that the positive rates for CD29, Scal-1 and CD45 were 97.1%,87.1%, 0.7%, respectively. The osteogenic differentiation of MSCs was verified by alkaline phosphatase staining and alizarin red staining; the chondrogenic differentiation of MSCs was identified by alcian blue staining. It is concluded that high-purity MSCs can be obtained by using the whole marrow adherence method and have multipotential differentiation.