[Determination of selenium in whole blood by microwave digestionatomic fluorescence spectrometry].

OBJECTIVE: To establish a rapid, simple, and accurate method for measuring selenium in whole blood by atomic fluorescence spectrometry. METHODS: The whole blood samples were treated by microwave digestion and the acids were dispelled. After that the samples were pretreated with 50% hydrochloric acid as a reducing agent. Then the content of selenium in the pretreated samples was measured by hydride generation atomic fluorescence spectrometry. RESULTS: The calibration curve for selenium is linear in the range of 0~60 μg/L (r=0.9999). The detection limit, relative standard deviation, and recovery rate were 0.133 μg/L, 1.22%~2.08%, and 96.5%~101.8%, respectively. CONCLUSION: This method is simple, with less consumption of chemical reagents, less pollution, and enough accuracy and sensitivity for determination of selenium in whole blood samples.