2009•Unpublished venueRequires access
Effect of A1a-Gln on functional Fas Ligand expression of human colon cancer cell
Zhigang Wu
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Abstract
Objective:To study the effect of A1a-Gln on Fas Ligand mRNA expression and apoptosis of lymphocyte induced by human colon cancer cells line SW480 with cell culture.Methods:Different concentrations of Ala-Gln were added in the cultured human colon cancer cells line SW480.The changes of Fas Ligand mRNA was detected by Real-Time reverse transcription polymerase chain reaction method(Real-Time RT-PCR)and apoptosis rate of lymphocyte induced by SW480 cell was measured by flow cytometry analysis.Results:RT-PCR revealed that the higher concentration A1a-Gln down-regulated Fas Ligand mRNA expression on SW480 cell(P0.05);flow cytometry showed that the higher concentration Ala-Gln reduced apoptosis rate of lymphocyte induced by SW480 cell(P0.05).The comparisons with different concentrations teams of Ala-Gln had statistical significance(P0.05).Conclusion:Ala-Gln in certain concentration can down-regulated Fas Ligand mRNA expression of human colon cancer cells line SW480.At the same time,the down-regulative effect can inhibit SW480 cells to counterattack lymphocyte.