Effect of A1a-Gln on Fas Ligand gene expression and apoptosis of lymphocyte induced by human colon carcinoma cell.

Objective To study the effect of A1a-Gln on Fas Ligand mRNA expression and apoptosis of lymphocyte induced by human colon cancer cells line SW480 with cell culture. Methods Different concentrations of Ala-Gln were added in the cultured human colon cancer cells line SW480.The changes of Fas Ligand mRNA was detected by the reverse transcription polymerase chain reaction method(RT-PCR)and apoptosis rate of lymphocyte induced by SW480 cell was measured by flow cytometry analysis. Results RT-PCR revealed that the higher concentration A1a-Gln down-regulated Fas Ligand mRNA expression on SW480 cell(P0.05); flow cytometry showed that the higher concentration Ala-Gln reduced apoptosis rate of lymphocyte induced by SW480 cell(P0.05). The comparisons with different concentrations teams of Ala-Gln have statistical significance(P0.05). Conclusion Ala-Gln in certain concentration can down-regulated Fas Ligand mRNA expression of human colon cancer cells line SW480. For the same time, the down-regulative effect can inhibit SW480 cells to counterattack lymphocyte.