Protection of QingYiII against liver injury in severe acute pancreatitis rats

Objective To explore the role of QingYi Ⅱ in Severe Acute Pancreatitis(SAP) with liver injury.Methods Twenty-four SD rats were divided to three groups:sham group(group A),severe acute pancreatitis group(group B),treatment group with QingYiⅡ(group C).The rats in group A were only subjected to obdominal opening.Rat SAP model was induced by retrograde injection of 3% sodium taurocholate to pancreatic duct.After the model successfully established,no treatments were given in goup B,in group C,QingYiⅡwas given.The rats were killed 24 h after operation,blood sample and pancreatic and liver tissues were collected.IL-1,IL-6,IL-8 was assayed by ELISA.Meanwhile,blood serum amylase(AMY),AST,ALT and LDH levels were determined using automated analyzer.Other tissues were put into a 10% formaline solution for routine light microcopic exeamination.Results Compared with group A,at the same time,the serum of IL-1,IL-6,IL-8 of group B increased sharply(P0.05),the serum of AST,ALT and LDH of group B increased sharply(P0.01),in group C,when QingYiⅡ was given,plasma level of AST,ALT,LDH decreased(P0.01).Conclusion QingYiⅡcan reduce pathological damage liver by decreasing the release of the inflammatory mediators.