Expression of CD44 in Liver Tissues of Rats with Severe Acute Pancreatitis and Protective Effect of Qingyi II Granules

Objective: To explore the role of CD44 in pancreas and liver damage in severe acute pancreastitis(SAP),and to investigate the effects of Chinese medicine Qingyi Ⅱ granules on the expression of CD44 and its mechanism.Methods: A total of 36 healthy SD rats were randomly divided into three groups: sham-operated group(group A),SAP model group(group B) and treatment group(group C).SAP model was established by retrograde injection of 30 g/L sodium taurocholate into bile-pancreas duct.Qingyi Ⅱ Granules 250 g/L was administered by gavage every 6 hours in group C.While the other two groups were infused with same volume of saline instead.All animals were sacrificed after modeling for 24 h.The CD44 expressions in pancreas and liver tissues were detected with immunohistochemistry method.Serum amylase(AMY),aspartate aminotransferasea(AST) and alanine aminotransferase(ALT) were tested.Pathologic changes of pancreas and liver tissues were observed under microscope.Results: Pancreas and liver tissues were damaged seriously in group B,and the expression of CD44 was positive,the serum levels of AMY,AST and ALT were significantly higher than those of groups A and C(P0.05).The expression level of CD44 was lower and activities of serum AMY,AST and ALT were higher significantly in group C than those in group B.(P0.05).Conclusions: The expression of CD44 may play an important role in pancreas and liver injury in SAP rats.Chinese medicine Qingyi Ⅱ granules could depress the expressions of CD44 in pancreas and liver tissues,and alleviate the pathologic damage of pancreas and liver in SAP rats.