Changes of acute renal injury in SAP rats and the protective function of QingYi II

Objective To observe the effects and explore the mechanism of the traditional Chinese medicine QingYi Ⅱ for severe acute pancreatitis(SAP) complicated with acute renal injury(ARI).Methods 48 healthy SD rats were randomly divided into three groups: sham operation group(group A,n = 16),SAP model group(group B,n = 16) and QingYi Ⅱ group(group C,n = 16).The model of SAP was induced by retrograde injection of 3% sodium taurocholate into bilio-pancreatic duct in group B,C,while group A were only flipped the intestinal canal;after one hour,group A,B were fed with saline,QingYi Ⅱ was fed to rats in group C.All rats were sacrificed after SAP had been produced for 12 hours and 24 hours,the blood was drawn from portal vein.The concentrations of serumamy-amylase,ureanitro-gen,creatinine and the expression of renal tissue TNF-α mRNA were measured.Pathologic change of kidney and pancreas were observed under microscope.Results The concentrations of serumamylase,urea nitrogen,creatinine,the expression of renal tissue TNF-α mRNA were higher in group B than those in group A(P 0.05).The concentrations of serumamylase,urea nitrogen,creatinine,the expression of renal tissue TNF-α mRNA were lower in group C than those in group B(P 0.05).Group B had serious pathologic damage in both pancrea and kidney,more ascites and sapon spot.Group C had less pathologic damage in both pancrea and kidney,more ascites and sapon spot.Conclusion The traditional Chinese medicine QingYi Ⅱ may play an important role in treating SAP complicated with ARI.