Simultaneous determination of paeoniflorin and hesperidin in Yiwei oral solution by HPLC

Objective To develop a HPLC method for simultaneous determination of paeoniflorin and hesperidin in Yiwei(traditional Chinese medicines) oral solution.Methods The analysis was carried out with a Kromasil ODS-1(4.6 mm ×250 mm,5 μm) column and a mobile phase of acetonitrile-0.1%phosphoric acid solution(21:79).The flow rate was 1.0 mL·min-1 and column temperature was 35 ℃.The detection wavelength was set at 230 nm.Results The calibration curve was linear within the range of 7140 mg·L-1(r=0.999 6) and 6-120 mg·L-1(r=0.999 5) for paeonifloin and hesperidin respectively,the average recoveries were 98.5%(RSD=0.67%) and 98.7%(RSD=1.1%) for paeoniflorin and hesperidin respectively.Conclusions This method is convenient,accurate and reproducible for determination of paeoniflorin and hesperidin in Yiwei oral solution.