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2009•Zhongguo shiyan fangjixue zazhiRequires access

Determination of paeoniflorin and hesperidin in Antihernolysis Granule by RP-HPLC

Wanqing Li

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Abstract

Objective:To determine the contents of paeoniflorin and hesperidin in Antihernolysis Granule. Methods:A Tianhe C_ 18 Column was used with methanol-1% Phosphoric acid (35∶65) as the mobile phase at the flow rate of 1.0ml/min.The detecting wavelength was set at 270 nm. Results The linearity of paeoniflorin was good in the range of 0.198 4 ～ 0.992 μg (r= 0.997 9 ), The linearity of hesperidin was good in the range of 0.211 2 ～1.056 μg(r= 0.999 3 ).The average recovery of paeoniflorin was 99.08%, RSD=1.47%(n=6).The average recovery of hesperidin was 100.67%, RSD=1.60%(n=6). Conclusion: The method is simple, reliable, accurate, and can be applied as the quality control method of Antihernolysis Granule.

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Objective:To determine the contents of paeoniflorin and hesperidin in Antihernolysis Granule. Methods:A Tianhe C_ 18 Column was used with methanol-1% Phosphoric acid (35∶65) as the mobile phase at the flow rate of 1.0ml/min.The detecting wavelength was set at 270 nm. Results The linearity of paeoniflorin was good in the range of 0.198 4 ～ 0.992 μg (r= 0.997 9 ), The linearity of hesperidin was good in the range of 0.211 2 ～1.056 μg(r= 0.999 3 ).The average recovery of paeoniflorin was 99.08%, RSD=1.47%(n=6).The average recovery of hesperidin was 100.67%, RSD=1.60%(n=6). Conclusion: The method is simple, reliable, accurate, and can be applied as the quality control method of Antihernolysis Granule.

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Available abstract

Objective:To determine the contents of paeoniflorin and hesperidin in Antihernolysis Granule. Methods:A Tianhe C_ 18 Column was used with methanol-1% Phosphoric acid (35∶65) as the mobile phase at the flow rate of 1.0ml/min.The detecting wavelength was set at 270 nm. Results The linearity of paeoniflorin was good in the range of 0.198 4 ～ 0.992 μg (r= 0.997 9 ), The linearity of hesperidin was good in the range of 0.211 2 ～1.056 μg(r= 0.999 3 ).The average recovery of paeoniflorin was 99.08%, RSD=1.47%(n=6).The average recovery of hesperidin was 100.67%, RSD=1.60%(n=6). Conclusion: The method is simple, reliable, accurate, and can be applied as the quality control method of Antihernolysis Granule.

Key concepts: Paeoniflorin, Hesperidin, Granule (geology), Chromatography, Chemistry, Phosphoric acid, High-performance liquid chromatography, Naringin

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