2007•Anhui Medical and Pharmaceutical JournalRequires access

Determination of paeoniflorin in zhengchaihuyin capsules by HPLC

Sun Jun-ye

Abstract

Aim To establish a method for determination of paeoniflorin in zhengchaihuyin capsules.Method The analysis was performed on a Sim pack CLC-C18 column,with a mobile phase of acetonitrile-0.1% phosphoric acid solution(13∶ 87),the detection wavelength was 230 nm.Result The linear range of paeoniflorin was between 21.2 and 106.0 mg ·L-1(r=0.999 2).Recovery of paeoniflorin was 100.68%,and RSD was 0.81%.Conclusion This method is simple,repeatable,and can be used for the quantitative analysis of paeoniflorin in zhengchaihuyin capsules.

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Key concepts: Paeoniflorin, Chromatography, Phosphoric acid, Chemistry, High-performance liquid chromatography, Linear range, Acetonitrile, Quantitative analysis (chemistry)