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2010•Research and Practice on Chinese MedicinesRequires access

Determination of Paeoniflorin from Different Germplasms of Peony

Liao Zi-rong

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Abstract

Objective To compare the content of paeoniflorin from different germplasms Paeonia lactifolra.Methods HPLC method was used on LichroCART RP-C18 column(4 mm×250 mm,5 μm)with HCN- 0.1% phosphoric acid(V:V=14:86) as mobile phase,the flow rate was 1.0 mL/min,the detection wavelength was at 230 nm and column temperature was 30 ℃.Results There was great difference that Paeoniflorin in different germplasms was quite different.Determinated of peoniflorin in different parts of Radix Paeonia Alba reveals was in terms of content of peoniflorin: rhizomafi brous rootaxial root.Conclusion The difference of peoniflorin content was mainly from germplasms source of Paeonia lactiflora .

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Objective To compare the content of paeoniflorin from different germplasms Paeonia lactifolra.Methods HPLC method was used on LichroCART RP-C18 column(4 mm×250 mm,5 μm)with HCN- 0.1% phosphoric acid(V:V=14:86) as mobile phase,the flow rate was 1.0 mL/min,the detection wavelength was at 230 nm and column temperature was 30 ℃.Results There was great difference that Paeoniflorin in different germplasms was quite different.Determinated of peoniflorin in different parts of Radix Paeonia Alba reveals was in terms of content of peoniflorin: rhizomafi brous rootaxial root.Conclusion The difference of peoniflorin content was mainly from germplasms source of Paeonia lactiflora .

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Available abstract

Objective To compare the content of paeoniflorin from different germplasms Paeonia lactifolra.Methods HPLC method was used on LichroCART RP-C18 column(4 mm×250 mm,5 μm)with HCN- 0.1% phosphoric acid(V:V=14:86) as mobile phase,the flow rate was 1.0 mL/min,the detection wavelength was at 230 nm and column temperature was 30 ℃.Results There was great difference that Paeoniflorin in different germplasms was quite different.Determinated of peoniflorin in different parts of Radix Paeonia Alba reveals was in terms of content of peoniflorin: rhizomafi brous rootaxial root.Conclusion The difference of peoniflorin content was mainly from germplasms source of Paeonia lactiflora .

Key concepts: Paeoniflorin, Paeonia lactiflora, Chemistry, High-performance liquid chromatography, Chromatography, Phosphoric acid, Radix (gastropod), Significant difference

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