ScholarLens Back to search
2006•Journal of Chengdu University of Traditional Chinese MedicineRequires access

Measurement of Contents of Paeoniflorin in the Different Fertility of Soil of Paeonia lactiflora by HPLC

Shu-Xin Chen

Open publisher page 0 citations

Abstract

Objective:To compare the contents of paeoniflorin in the different fertility of soil of paeonia lactiflora.Method:The contents of paeoniflorin determined by HPLC.Chromatographic condition included Spherisorb 10 ODS1(4.6 mm×250 mm,10?μm) and the mobile phase consisting of a mixture of methanol-0.05%mol/LKH_2PO_3(40∶65).Paeoniflorin was detected at 230 nm.The standard curve was linear in the range of 2.5～12.5?μg with correlation coefficient 0.99998.The average recovery of paeoniflorin was 100.5% with RSD=2.2%.Results:The contents of paeoniflorin were 9.01%,5.56%,9.69%,8.23 %,9.33%,6.22%,7.20% in the different fertility of soil respectively.Conclusion:The method is accurate quick and good reappearance,it can be used as the important basis of quality estimation in the culture of paeonia lactiflora.

About this research paper

What this paper is about

Objective:To compare the contents of paeoniflorin in the different fertility of soil of paeonia lactiflora.Method:The contents of paeoniflorin determined by HPLC.Chromatographic condition included Spherisorb 10 ODS1(4.6 mm×250 mm,10?μm) and the mobile phase consisting of a mixture of methanol-0.05%mol/LKH_2PO_3(40∶65).Paeoniflorin was detected at 230 nm.The standard curve was linear in the range of 2.5～12.5?μg with correlation coefficient 0.99998.The average recovery of paeoniflorin was 100.5% with RSD=2.2%.Results:The contents of paeoniflorin were 9.01%,5.56%,9.69%,8.23 %,9.33%,6.22%,7.20% in the different fertility of soil respectively.Conclusion:The method is accurate quick and good reappearance,it can be used as the important basis of quality estimation in the culture of paeonia lactiflora.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To compare the contents of paeoniflorin in the different fertility of soil of paeonia lactiflora.Method:The contents of paeoniflorin determined by HPLC.Chromatographic condition included Spherisorb 10 ODS1(4.6 mm×250 mm,10?μm) and the mobile phase consisting of a mixture of methanol-0.05%mol/LKH_2PO_3(40∶65).Paeoniflorin was detected at 230 nm.The standard curve was linear in the range of 2.5～12.5?μg with correlation coefficient 0.99998.The average recovery of paeoniflorin was 100.5% with RSD=2.2%.Results:The contents of paeoniflorin were 9.01%,5.56%,9.69%,8.23 %,9.33%,6.22%,7.20% in the different fertility of soil respectively.Conclusion:The method is accurate quick and good reappearance,it can be used as the important basis of quality estimation in the culture of paeonia lactiflora.

Key concepts: Paeoniflorin, Paeonia lactiflora, Chromatography, High-performance liquid chromatography, Correlation coefficient, Chemistry, Linear correlation, Linear relationship

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Measurement of Contents of Paeoniflorin in the Different Fertility of Soil of Paeonia lactiflora by HPLC — Research Paper | ScholarLens