Determination of Paeoniflorin and Albiflorin in Different Germplasms and Parts of Peony Root

Objective To compare the content and distribution of paeoniflorin and albiflorin in different germplasms and parts of peony root under the same analysis. Methods The HPLC method was taken by using Lichro CART RP-C18(4 mm×250 mm,5 μm)column with HCN-0.1% phosphoric acid(V:V=14:86) as mobile phase,flowing at 1.0 mL·min-1,with the detection wavelength as 230 nm,column temperature at 30 ℃ and loading volume as 10 μL. Results Paeoniflorin and albiflorin in different germplasms were quite different.It was showed that rhizomafibrous rootaxial root in terms of peoniflorin contained in peony root;fibrous rootrhizomaaxial root in terms of albiflorin in different parts of radix paeonia alba. Conclusion The main reason for content difference of paeoniflorin and albiflorin was germplasms.There is small variation for paeoniflorin and albiflorin in geo-authentic product area of germplasm.