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2010•Dalian Gongye Daxue xuebaoRequires access

Analysis of Epimedium Flavonoids by HPLC

LI Dan-feng

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Abstract

The analysis method was established for evaluating Epimedium Flavonoids by HPLC,which was performed on a Sinochrom ODS-BP column,with acetonitrile and water as mobile phase.The gradient elute ratio of acetonitrile were: 0-12 min(20%-30%),12-20 min(30%-36%),20-30 min(36%-58%).The flow rate was 1.0 mL/min.The detection wavelength was 270 nm.The content of 13 kinds of flavonoids in the water extract of Epimedium were determined based on Icariin.The result showed that the method was simple,specific and accurate which can be used for controlling the quality of the water extract of Epimedium.

About this research paper

What this paper is about

The analysis method was established for evaluating Epimedium Flavonoids by HPLC,which was performed on a Sinochrom ODS-BP column,with acetonitrile and water as mobile phase.The gradient elute ratio of acetonitrile were: 0-12 min(20%-30%),12-20 min(30%-36%),20-30 min(36%-58%).The flow rate was 1.0 mL/min.The detection wavelength was 270 nm.The content of 13 kinds of flavonoids in the water extract of Epimedium were determined based on Icariin.The result showed that the method was simple,specific and accurate which can be used for controlling the quality of the water extract of Epimedium.

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Available abstract

The analysis method was established for evaluating Epimedium Flavonoids by HPLC,which was performed on a Sinochrom ODS-BP column,with acetonitrile and water as mobile phase.The gradient elute ratio of acetonitrile were: 0-12 min(20%-30%),12-20 min(30%-36%),20-30 min(36%-58%).The flow rate was 1.0 mL/min.The detection wavelength was 270 nm.The content of 13 kinds of flavonoids in the water extract of Epimedium were determined based on Icariin.The result showed that the method was simple,specific and accurate which can be used for controlling the quality of the water extract of Epimedium.

Key concepts: Icariin, Epimedium, Chromatography, Chemistry, High-performance liquid chromatography, Acetonitrile, Gradient elution, Traditional medicine

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