2011•Tianjin Chemical IndustryRequires access

Determination of icariin in supercritical fluid extracts of epimedium by RP-HPLC

Xiaoli Niu

Abstract

Objective:To determine the content of icariin in supercritical fluid extracts of Epimedium by RP-HPLC.Methods:The sample was separated on a C18 column(4.6 mm×250 mm,5 μm) with the mobile phase of methanol-0.1 % acetic acid in water solution as gradient eluent.The flow rate was 1 mL·min-1 and the detection wavelength was set at 270 nm.Results:The calibration curve was linear in the range of 0.212~2.12 μg for icariin;The average recovery was 98.7%,RSD=1.18%(n=6).Conclusion: The method is rapid and accurate.It can be used as a method of quality control for supercritical fluid extracts of epimedium.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Icariin, Epimedium, Chromatography, Chemistry, Calibration curve, High-performance liquid chromatography, Supercritical fluid, Supercritical fluid chromatography