Method Evaluation on The Vanadate Oxidation for Determination of Total Bilirubin and Direct Bilirubin

Objective Method comparison of Wako vanadate oxidation with improved J-G method for determination of bilirubin.Methods We use the Wako vanadate oxidation reagent of bilirubin to determinate serum total bilirubin and direct bilirubin simultaneously,take a comparison with the method of improved J-G and also take run-in-run test,linear test,recovery test,interfere test of vanadate oxidation.Results Vanadate oxidation( Y )has good correlation with improved J-G (total bilirubin Y =0.992 6 X +2.234 4, r =0.999 1;direct bilirubin Y =0.996 8 X +1 169 4, r =0.998 2).Linear range of total bilirubin can attach to 684 μmol/L and direct bilirubin can attach to 342 μmol/L.The inter day CV for total bilirubin is 1.17% and direct bilirubin is 1.88%.The intra day CV for total bilirubin is 1.88% and direct bilirubin is 2.76%.Hemolyte,lipidemia and vitamin C have rarely interfere.The average recovery rate of total bilirubin is 98.7%,direct bilirubin is 102%.Conclusion Vanadate oxidate method is accurate and sensitive and it can be used in the routine determination of bilirubin.