Drug resistance and distribution of pathogens isolated from blood culture

Objective To investigate the distribution and drug resistance of pathogens isolated from positive blood culture so as to provide evidence for rational use of antibiotics in clinical practice. Methods The blood cultures of patients from Jan 2013 to Dec 2013 were collected. The bacteria identification and drug sensitivity test were performed,and the results were statistically analyzed by using WHONET 5. 6 software. Results In the total 2,017 blood culture specimens,there were 370 strains( 18. 34%) of pathogens,including 142 strains( 38. 38%) of gram-positive bacteria,213 strains( 57. 57%) of gram-negative bacteria,and 15 strains( 4. 05%) of fungi. The top five pathogens were coagulase-negative Staphylococcus( CNS),Escherichia coli,Klebsiella pneumoniae,Enterococcus and Staphylococcus aureus,accounting for 19. 73%,17. 03%,13. 51%,6. 22% and 4. 59%,respectively. Gram-negative bacteria were highly resistant to all the antibiotics except that Escherichia coli and Pseudomonas aeruginosa were sensitive to piperacillin / tazobactam. The detection rates of extended-spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae were 46. 80% and 20. 00% respectively. Gram-positive bacteria were 100% sensitive to Linezolid. MRSA strains accounted for 25. 00%,and MRCNs accounted for 92. 00%.There were one strain of Staphylococcus epidermidis and four strains of Enterococcus faecalis,all resistant to Vancomycin. Conclusion The detection rate of the pathogens isolated from blood culture is relatively low,with gramnegative bacteria being predominant. The drug resistance rate is relatively high,and vancomycin-resistant Staphylococcus epidermidis and Enterococcus faecalis had appeared. It is of great significance to reduce the incidence of nosocomial infections and guide the reasonable use of antibiotics through understanding the distribution and drug resisitance of the pathogens causing bloodstream infections.