Extraction of Total Flavonoids from Sanguisorba officinalis Root by Pressurized Liquid Extraction

The extraction process of flavonoids from Sanguisorba officinalis root using pressurized liquid extraction(PLE) was studied and compared with reflux extraction.Significant parameters affecting PLE procedure such as the concentration of ethanol,solid-liquid ratio, temperature,pressure,time,and material size were discussed and optimized by the orthogonal test.The results showed that the optimized conditions were as follows:the extraction solvent 60% ethanol water solution,the material size 40 mesh,ratio of mass of raw material to volume of extraction solvent 1:20,extraction temperature 120 ℃,extraction 30 min under 5.0 kg/cm2 of pressure.The recovery of extraction could reach 17.35%.Comparing with reflux extraction,PLE can obviously increase the recovery of extraction.