Study on Extraction Technique of Total Flavonoids from Scorzonera Divaricata

The extraction technique of total flavonoids from Socrzonera divricata by uniform design was studied.The effects of ethanol concentration,extraction time,the ratio of material to solvent and extraction number were observed.The result showed that the best extraction technique of water bath reflux method was as follows:15% ethanol,the ratio of material to solvent of 1:34,extraction time of 45min,and extraction for 3 times.The best extraction technique of ultrasonic-microwave synergistic was as follows:30% ethanol,the ratio of material to solvent of 1:36,extraction time of 41min,and extraction for 3 times.By comparison,ultrasonic-microwave method has advantages of saving time,lower energy consumption and facility of manipulation.