Extraction of Total Flavonoids from Frutus aurantii immaturus by Pressurized Liquid Extraction

The extraction process of flavonoids from Frutus aurantii immaturus using pressurized extraction(PE) was studied and compared with reflux extraction.Parameters affecting PE procedure such as the concentration of ethanol,solid-liquid ratio,temperature,pressure,time,and material size were discussed and optimized by orthogonal test.The results show that the optimized conditions are as follows: the extraction solvent is 40%(v /v)ethanol aqueous solution,the material size is 60 mesh,ratio of mass of raw material to volume of extraction solvent is 1∶ 20,extraction temperature is 130 ℃,extraction for 20 min under pressure of 500 kPa.The recovery of extraction could reach 4.16 ﹪ with the extraction rate of 2.08 mg /(g·min).With reflux extraction under the same solid-liquid ratio of extraction solvent,the recovery of extraction is 3.44% with the extraction rate of 0.19 mg /(g·min).PE can obviously increase the recovery of extraction.