Analysis of drug resistance and distribution of pathogens isolated from blood cultures

Objective To investigate the distribution and antibiotic resistance of pathogens isolated in blood culture from the inpatients, which can be used to guide clinical therapy. Methods Bact/ALERT 3D System was used to perform blood culture,and VITEK 2 Compact Instrument was used to identify Bacteria and to test antimicrobial susceptibility. Results Among 7 716 blood culture samples, the rate of positive cultures was 8.97% (692/7 716). Moreover,395 strains were Gram-negative bacteria (57.08%), 278 strains were Gram-positive bacteria (40.17% ) and 19 strains were fungi (2.75%). Escherichia coli,Coagulase negative Staphylococcus, Klebsiella pneumoniae, Staphylococcus aureus and Enterococcus were the most common isolates. The incidences of meticillin-resistant S. aureus (MRSA) and meticillin-resistant coagulase negative Staphylococcus (MRCNS) were 20% and 83.4% , respectively. No vancomycin and linezolid resistant Staphylococcus strains were found. There were 7.7% of Enterococcus resistant to vancomycin. No linezolid and tigecycline resistant Enterococcus were found. Enterobacteriaceae isolates were highly sensitive to carbapenem. 45.0% of Escherichia coli and 22.9% of Klebsiella pneumoniae isolates were the strains producing extended spectrum-β-lactamases (ESBLs). Non-fermenting bacteria showed a relatively high resistance to many antimicrobial drugs. Conclusions There are many different kinds of pathogens isolated from blood culture ,which showed high resistance to many antimicrobial agents.Clinical doctors should pay great attention to the blood culture and rationally use of antibiotics according to drug susceptibility test result.