Distribution and drug resistance of isolated bacteria from blood culture in general hospital from 2007 to 2010

OBJECTIVE To understand and investigate the distribution and drug resistance of pathogens cultured from blood from 2007 to 2010 so as to provide the clinical evidence for rational use of antimicrobial drugs.METHODS BacT/ALERT3D automated blood culture instrument was adopted,positive samples were transferred to blood agar and chocolate plates.Bacteria identification and susceptibility testing were performed with ATB expression instrument identification system.Totally 4741 copies of blood cultures were retrospectively analyzed with software WHONET 5.4.RESULTS Totally 613 pathogens were isolated with the positive rate of 12.93%,among which there were 368 strains(60.03%) of gram-positive cocci,171 strains(27.90%) of gram-negative bacilli,74 strains(12.07%) of fungi.The drug resistance rates of Staphylococcus to linezolid,vancomycin,and nitrofurantoin were 0.The susceptibility of gram-negative bacteria(except for Acinetobacter baumannii) to carbapenems was relatively high.Methicillin-resistant isolates accounted for 92.86% among coagulase-negative Staphylococcus;methicillin-resistant isolates accounted for 61.54% among S.aureus.The detection rates of extended spectrum β-lactamases-producing Escherichia coli and Klebsiella pneumoniae were 22.50% and 20.00% respectively.CONCLUSION The pathogens detected in positive blood cultures are mainly coagulase-negative Staphylococci.The resistance rates of many strains are high,which should be highlighted.