SSR Analysis of M_1 Variation of Dry Seeds Implanted by ~7Li Ion Beam in Wheat

Dry seeds of two wheat cultivars Xinmai18 and Lunxuan987 were implanted by different doses of 7Li ion beam to study the effects on seedling growth and DNA variation in M1 generation.The results showed that 7Li ion beam implantation into wheat seeds can significantly affect seedling height,while no significant effect on germination rate and root growth.The 20 Gy dose of 7Li ion beam implantation could make the seedling height about 50% shorter than the control.The bands of SSR were greatly changed and the average polymorphic frequency was both 13.1% in two wheat cultivars implanted by different doses of 7Li ion beam.The polymorphic patterns were mainly the increase or deletion of amplified fragments.It was concluded that high biological damage effects and DNA variations could be induced by 7Li ion beam implantation,which could result in the hereditable variation in the later generation.