Transferring Corn DNA into Wheat Via Ion Beam Mediated Method
Feng Wei-sen
Abstract
Feng Wei-sen
Abstract
In the experiment,6 corn varieties were taken as the donors and 4 wheat varieties as the acceptor,and the transformation of hereditary materials were completed by ion beam mediated technique.The characteritics of the materials in M1 were identified.The results showed that the seedling frequency of wheat after ion beam implantation was lowered,which was one of the biological effects of low energy ion beam.In early and mid growing stages,the main agronomic characters of the materials did not display obvious differences.In late stages,most of the agronomic characters had no differences from CK except the growth vigour,ear shape,tillering state and single ear variation.In CK2-1—CK2-4 and CK3-1—CK3-4,there was also mutation,but the mutation number was few and the mutation frequency was low.The biological effects of ion beam treatment was apparent,with more mutation plants and higher variation frequence.
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In the experiment,6 corn varieties were taken as the donors and 4 wheat varieties as the acceptor,and the transformation of hereditary materials were completed by ion beam mediated technique.The characteritics of the materials in M1 were identified.The results showed that the seedling frequency of wheat after ion beam implantation was lowered,which was one of the biological effects of low energy ion beam.In early and mid growing stages,the main agronomic characters of the materials did not display obvious differences.In late stages,most of the agronomic characters had no differences from CK except the growth vigour,ear shape,tillering state and single ear variation.In CK2-1—CK2-4 and CK3-1—CK3-4,there was also mutation,but the mutation number was few and the mutation frequency was low.The biological effects of ion beam treatment was apparent,with more mutation plants and higher variation frequence.
Key concepts: Seedling, Ion beam, Mutation, Agronomy, Ion, Transformation (genetics), Beam (structure), Mutation breeding