SSR Identification of M_3 Mutants and Study on TheirIsolation in Ar Ion Implanted Wheat
Hou Da-peng
Abstract
Hou Da-peng
Abstract
Dry seeds of HuaPei 5 Wheat were implanted by ion beam of Ar+.The results showed that the growth date and agricultural characters of M1 had no significant difference compared with those of CK.In M2,there were variation forms of plant height and spike length.The variation rate was 0.2%.The SSR identification of 10 mutations showed that there were some special bands of PCR production using 6 primer pairs screened with 90 primer pairs.The variation points were in 2A,2D,3B,5A aegricorpus,respectively.In M3,10 variation plants isolated strongly,positive or negative,it was beneficial to find special variation forms.
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Dry seeds of HuaPei 5 Wheat were implanted by ion beam of Ar+.The results showed that the growth date and agricultural characters of M1 had no significant difference compared with those of CK.In M2,there were variation forms of plant height and spike length.The variation rate was 0.2%.The SSR identification of 10 mutations showed that there were some special bands of PCR production using 6 primer pairs screened with 90 primer pairs.The variation points were in 2A,2D,3B,5A aegricorpus,respectively.In M3,10 variation plants isolated strongly,positive or negative,it was beneficial to find special variation forms.
Key concepts: Primer (cosmetics), Biology, Variation (astronomy), Mutant, Significant difference, Horticulture, Animal science, Botany