DISTANT MOLECULAR HYBRID DERIVED FROM SOYBEAN AND WHEAT WITH ION IMPLANTATION AND VARIATION ANALYSIS

The exogenous total DNAS of soybean were introduced into wheat breeds, Wan9210 and Yanmai 5, by Ar + implantation. The results showed that the dose of ions implanted was one of the important factors affected the germination and survival rate of wheat. The suitable dose was 7×10 16 ions/cm 2. The plants treated with Ar + and soybean DNA didn't show any apparent variation in the first generation. After harvest, the protein content of transformated progeny was analyzed. The protein content of two signle plants reached 20 46% and 25 35% respectively, which were much higher than that of control. It is concluded that low energy of ion beam mediated DNA delivery into mature seeds was a simple and effective method for the genetic material exchange of species with distant genetic relationship.