2012•Zhongguo shiyan fangjixue zazhiRequires access
Optimization of Purification Technology for Total Phenolic Acid from Mentha haplocalyx by Macroporous Resin
Bin Liu
Open publisher page 1 citations
Abstract
Objective:To optimize purification technology of total phenolic acid from Mentha haplocalyx by macroporous resin.Method: With the content of total phenolic acid and adsorption-resolution rate as indexes,optimum resin model was determined by investigating adsorption capacity of five different macroporous resin on total phenolic acid,and optimized its purification technology conditions.Result: HPD-400 macroporous resin was selected,Optimum purification conditions were as follow:The concentration of sample liquid was 0.1 g · mL-1 and then turn to pH 3 with acetic acid,the amount of sample 6 BV,ratio of diameter-height 1∶9,2 BV 20% ethanol to wipe off impurity with flow velocity of 2 BV · h-1,3 BV 70% ethanol to elute with 4 BV · h-1,eluate collected,purity of total phenolic acid 53%.Conclusion: Optimized purification technology was fit for separating and easy to operate with good reproducibility,it could be used to separate and enrichment total phenolic acid from M.haplocalyx.