Optimization of Purification Technology for Total Flavonoids from Leontopodium leontopodioid

Objective:To optimize enrichment and purification technology of total flavonoids from Leontopodium leontopodioid by macroporous resin.Method: Optimum macroporous resin and its purification technology was optimized by static adsorption/desorption and dynamic adsorption/desorption.The content of total flavonoids was determined by ultraviolet and visible spectrophotometry method.Result: HPD100 macroporous resin showed good concentration and purification capabilities to total flavonoids from L.leontopodioid.Optimum technology conditions were as follows: 2 BV sample liquid(the concentration of raw drug was 0.1 g·mL-1) of L.leontopodioid was passed through HPD-100 resin in 2 h with adsorption rate of 1 BV·h-1,eluted by 5 BV water,4 BV 30% ethanol,3 BV 50% ethanol,respectively,elution flow rate was 4 BV·h-1.Eluate was collected to concentrating under vacuum.Under this optimized technology,the elution rate of total flavonoids was more than 90%.Conclusion: This optimized technology was stable,feasible,it was suitable for industrial production.