Optimization of Purification Process for Total Flavonoids from Glycyrrhiza uralensis by Macroporous Adsorption Resin

Objective:To optimize separation and purification technology of total flavonoids from Glycyrrhiza uralensis by macroporous adsorption resin.Method: Twelve different types of resin were selected with the amount of absorption and desorption ratio of total flavonoids as indexes,based on this,separation and purification technology of total flavonoids were optimized.Result: Purification effect of AB-8 type macroporous was the best,its optimum technology conditions were:the concentration of sample solution 0.2 g·mL-1,maximum sample quantity 2 mL to 1 mL resin,velocity of absorption 0.5 BV·h-1,pH of sample solution 6,6 BV water to remove impurities at 1 BV·h-1,eluted by 4 BV 70% ethanol at flow rate of 1 BV·h-1.Under this optimized process,purity of total alkaloids in elution was 38%.Conclusion: This optimized process was rational,feasible and suitable for industrial production.