Optimization of Purification Technology for Total Flavonoids from Mentha haplocalyx

Objective:To optimize polyamide purification technology of total flavonoids from Mentha haplocalyx.Method:With the content and transfer rate of total flavonoids as indexes,adsorption capacity of 5 different kinds of purification materials for total flavonoids was investigated by single factor test,in order to determine optimum purification material,and adsorption,impurity removing and eluting conditions in purification technology were optimized.Result:Optimum purification conditions were as follows:The concentration of sample solution 0.1 g·mL-1,diameter-height ratio of column 1:9,adsorption rate 2 BV·h-1,proportion between the total amount of solution and resin column 1:6,3 BV of 10% ethanol to wipe off impurity with flow rate of 4 BV·h-1,then 3 BV of 90% ethanol to elute with 8 BV·h-1.After purified by polyamide(30-60 mesh),purity of total flavonoids was up to 50%.Conclusion:This optimized purification technology was simple and feasible with good reproducibility,it could be used for the separation and the enrichment of total flavonoids from M.haplocalyx.