Studies on Quality Control Standards of Qingweibaoan Pills

Objective To establish a method for quality control of Qingweibaoan Pills.Methods Rhizoma atractylodis macrocephalaer,Fructus crataegi,Fructus aurantii immaturus,Percarpium citiiri reticulatae viride,Radix et rhizome glycyrrhizae and Semen arecae in Qingweibaoan Pills were qualitatively identified by thin layer chromatography.The content of magnolol and honokio was determined by high performance liquid chromatography.Yilite C18(250 mm×4.6 mm,5 μm) was used as the chromatographic column and methanol-water(50∶50)as the mobile phase.The detection wavelength was 222 nm;the flow rate was 1.0 ml/min,and the column temperature was 40℃.Results The six identified herbs showed the same color pot as the control herbs in the same position.The linear response range was from 0.0470-0.4696 μg,0.0295-0.2952 μg of magnolol(r=0.9999,n=6).The average recovery of magnolol and honokio was 98.89%,101.94%,and RSD was 0.98%,0.96% respectively.Conclusion This method is sensitive,reliable and simple and can be used in qualify control of Qingweibaoan Pills.