Quality standard for Manyokin Tea

AIM To establish the quality standards for Manyokin Tea(Cassiae Semen,Aurantii Fructus immaturus,Magnoliae officinalis Cortex,Citri Reticulatae Pericarpium,etc.).METHODS Cassiae Semen,Aurantii Fructus immaturus and Magnoliae officinalis Cortex were identified by TLC.A RP-HPLC method was established to determine magnolol and honokiol in Manyokin Tea.The analytical column was DiamonsidTM-C18 column.The mobile phase was methanol-0.3% phosphoric acid(68∶ 32) with the flow rate of 1.0 mL/min.The detection wavelength was set at 294 nm.The column temperature was 30 ℃.RESULTS The TLC spots developed was clear.The linear range of magnolol was 44.4-111.0 μg/mL(r=0.999 3) and that of honokiol was 15.0-45.0 μg/mL(r=0.999 1).The RSDs of the method precision were 1.28% and 1.52%(n=6).The RSDs of stability in 12 h were 0.95% and 1.24%.The average content of magnolol was 0.88 mg/g(RSD was 1.83%),and honokiol was 0.33 mg/g(RSD was 2.30%) in repeatability test.The average recoveries of magnolol and honokiol were 99.2% and 96.2%,respectively.CONCLUSION This method is accurate and reliable,can be used for quality control of this preparation.