Cloning and Activity Analysis of Soybean SACPD-C Promoter

The SACPD-Cp promoter of soybean SACPD-C was isolated from the genomic DNA of soybean cultivar Jidou 2 using TAIL PCR.Promoter sequence analysis by PLACE showed that the cloned fragment contained many motifs that constituted the seed-specific cis-elements.Replacing CaMV35S promoter of pCAMBIA1301 with the SACPD-Cp fragment,the binary expression vector pCAM-SACPD-Cp was constructed.Transient expression by Agrobacterium tumefaciens mediated method,the histochemical GUS analysis and fluorometric GUS analysis were used for testing the expression of the GUS activity.The results indicated that GUS activity driven by SACPD-Cp fragment was 93.01% of that driven by CaMV35S promoter.The SACPD-Cp promoter did not have the homology compared with the reported promoters.GUS activity assays indicated that GUS was expressed only in seeds,but not in roots,stems and leaves,which suggests the SACPD-Cp is a seed-specific promoter.