2012•Jiangsu nongye xuebaoRequires access
Characterization of the promoter of a receptor like gene Gbvdr3 in island cotton.
Yongzhe Ren, Yuwen Yang, Rongfu Wang, Tianzi Chen, Zhang Baolong
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Abstract
In this study,the promoter region of a receptor like gene Gbvdr3 in Gossypium barbadense was obtained by genome walking,and the sequence was analyzed by PLACE software.The sequence analysis showed the promoter contained elements related to stress resistance and light regulation.The cloned promoter was fused to GUS reporter gene and was transformed into Arabidopsis.Eight transgenic plants were obtained by kanamycin sulfate screening and PCR method.GUS staining revealed the GUS expression was specifically detected in roots,flowers and seeds.The results indicated that the promoter of Gbvdr3 was a tissue-specific promoter.