Cloning and Expression Analysis of GmCYP78A5 Promoter

CYP78A5 promoter was isolated from soybean (Glycine max L. Merrill) plant by using PCR technology. DNA sequence alignment indicated that the amplified fragment (1650bp) was 99.21% homologous to the correspondent regions of the reported sequences. Bioinformatics analysis showed that the GmCYP78A5 promoter contains a lot of inducible or tissue-specific expression elements. RT-PCR results indicated that the gene GmCYP78A5 highly expressed in immature seed, weekly expressed in stem of soybean, but no expressed in root, leaf and flower. To further study the tissue expression patterns of GmCYP78A5 gene, the promoter of the gene GmCYP78A5 was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into tobacco (Nicotiana tabacum) by Agrobacterium-meditated method. The expression of the GUS gene in the transgenic tobacco plants indicated that the GmCYP78A5 promoter could drive the GUS reporter gene to express highly in the leaf, stem, sepal, pedicel, seeds of the transgenic tobacco plants, demonstrating that the expression patterns of the GmCYP78A5 promoters in soybean and tobacco were inconsistent.