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Cloning and Identification of the Root Specific Promoter from Soybean

Zhi Li

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Abstract

The promoter region of root-specific gene was isolated from the genomic DNA of soybean Jilin 17 by PCR method,and obtained the promoter fragment RSP,the length of the clone sequence is 2.5kb.The results of sequence analysis indicated that this fragment had 97% homology compared with the reported promoters.The cloned promoter was fused to the GUS reporter gene to construct plant expression vector pRSP-GUS,which was transferred into tobacco(Nicotiana tabacum)NC89 by Agrobacterium tumefaciens-mediated method and severa1 transformed plants were obtained.GUS activity assays of different soybean organs indicated that expression of GUS was active only in roots,which suggests the RSP gene is root-specific promoter.

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What this paper is about

The promoter region of root-specific gene was isolated from the genomic DNA of soybean Jilin 17 by PCR method,and obtained the promoter fragment RSP,the length of the clone sequence is 2.5kb.The results of sequence analysis indicated that this fragment had 97% homology compared with the reported promoters.The cloned promoter was fused to the GUS reporter gene to construct plant expression vector pRSP-GUS,which was transferred into tobacco(Nicotiana tabacum)NC89 by Agrobacterium tumefaciens-mediated method and severa1 transformed plants were obtained.GUS activity assays of different soybean organs indicated that expression of GUS was active only in roots,which suggests the RSP gene is root-specific promoter.

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Available abstract

The promoter region of root-specific gene was isolated from the genomic DNA of soybean Jilin 17 by PCR method,and obtained the promoter fragment RSP,the length of the clone sequence is 2.5kb.The results of sequence analysis indicated that this fragment had 97% homology compared with the reported promoters.The cloned promoter was fused to the GUS reporter gene to construct plant expression vector pRSP-GUS,which was transferred into tobacco(Nicotiana tabacum)NC89 by Agrobacterium tumefaciens-mediated method and severa1 transformed plants were obtained.GUS activity assays of different soybean organs indicated that expression of GUS was active only in roots,which suggests the RSP gene is root-specific promoter.

Key concepts: Agrobacterium tumefaciens, Promoter, Biology, GUS reporter system, Nicotiana tabacum, Gene, Molecular biology, Reporter gene

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