Determination of Hesperidin and Baicalin In Zhishidaozhi Wan by HPLC

Aim To develop an HPLC quantitative method for the determination of Hesperidin and Baicalin in Zhishidaozhi Wan. Methods The chromatographic conditions include column Intersil C_ 18 , mobile phase: acetonitrile-water(20∶80, which contains 0.7 % triethylamine that was adjusted to pH 2.5 by phosphoric acid) .The flow rate was 1.0 ml/min and monitored at 276 nm. Results the retention time of Hesperidin and Baicalin as 8.5 min and 15.6 min respectively. The number of theoretical plates calculated by Hesperidin and Baicalin peak as 2 500 and 2 600 respectively, The regress equation for Hesperidin was Y= 20 367 313.7X +3 946 464.7,r = 0.999 6,and the linear range was 0.480 ～ 2.880μg.The regress equation for Baicalin was Y= 63 795 699.4X+6 084 871.0,r= 0.999 6, and the linear range was 0.360～ 2.160μg. The average recovery of Hesperidin and Baicalin were 101.3%, RSD 1.6%(n=9)% and 99.5%, RSD 1.5%(n=9)% respectively. Conclusion The method was sensitive, time-saving and accurate for the determination of Hesperidin and Baicalin In Zhishidaozhi Wan .