HPLC determination of naringin,hesperidin and baicalin in Jiebiao Qingfei pills

Objective:To develop an HPLC method for determination of three constituents(naringin,hesperidin,baicalin)in Jiebiao Qingfei pills.Methods:Analysis was carried out on a ZORBAX SB C 18column(4.6 mm×250 mm,5 μm) eluted with water containing 0.05% phosphoric acid and 2% acetonitrile-acetonitrile as the mobile phase in the gradient elution at the flow rate of 1 mL·min-1.The UV detection wavelength was 277 nm,and the temperature of column was 40 ℃.The sample injection volume was 10 μL.The contents of naringin,hesperidin and baicalin were calculated by external standard method.Results:The linear ranges of naringin,hesperidin and baicalin were 5.26-52.5 μg·mL-1(r=0.9999),5.12-30.7 μg·mL-1(r=0.9999),and 10.0-60.2 μg·mL-1(r=0.9998),respectively;The average recoveries were 101.7%(RSD=0.54%),99.2%(RSD=1.3%),101.3%(RSD=1.0%),respectively.Conclusion:The HPLC method is simple,reliable and specific with good reproducibility,and can be used for determination of three constituents in Jiebiao Qingfei pills.