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2007•Strait Pharmaceutical JournalRequires access

HPLC determination of naringin,hesperidin and baicalin in Tongxuanlifei pill

Zheng Guang-dong

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Abstract

OBJECTIVE To establish a method for determining simultaneousty the contents of naringin,hesperidin and baicalin in Tongxuanlifei pill by HPLC.METHODS Applying HPLC with the analytical column BDSC_18(250mm×4.6mm,5μm),the mobile phase was composed of acetonitrile-0.1% phosphoric acid(20∶80);the flow rate was 1.0mL·min-1;the detection wavelength was at 280nm.RESULTS The range of fine linear relationship between the concentrations and peak areas was 10.04-100.4μg·mL-1(r=0.9998)for naringin,12.9-129μg·mL-1(r=0.9998)for hesperidin,17.44-174.4μg·mL-1(r=0.9997)for baicalin,the average recoveries of naringin,hesperidin and baicalin were 98.56%(RSD=1.30%),99.53%(RSD=0.92%)and 99.16%(RSD=1.32%)respectively.CONCLUSION The method is simple,accurate,and can be applied to the quantitative determination in this preparation.

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OBJECTIVE To establish a method for determining simultaneousty the contents of naringin,hesperidin and baicalin in Tongxuanlifei pill by HPLC.METHODS Applying HPLC with the analytical column BDSC_18(250mm×4.6mm,5μm),the mobile phase was composed of acetonitrile-0.1% phosphoric acid(20∶80);the flow rate was 1.0mL·min-1;the detection wavelength was at 280nm.RESULTS The range of fine linear relationship between the concentrations and peak areas was 10.04-100.4μg·mL-1(r=0.9998)for naringin,12.9-129μg·mL-1(r=0.9998)for hesperidin,17.44-174.4μg·mL-1(r=0.9997)for baicalin,the average recoveries of naringin,hesperidin and baicalin were 98.56%(RSD=1.30%),99.53%(RSD=0.92%)and 99.16%(RSD=1.32%)respectively.CONCLUSION The method is simple,accurate,and can be applied to the quantitative determination in this preparation.

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Available abstract

OBJECTIVE To establish a method for determining simultaneousty the contents of naringin,hesperidin and baicalin in Tongxuanlifei pill by HPLC.METHODS Applying HPLC with the analytical column BDSC_18(250mm×4.6mm,5μm),the mobile phase was composed of acetonitrile-0.1% phosphoric acid(20∶80);the flow rate was 1.0mL·min-1;the detection wavelength was at 280nm.RESULTS The range of fine linear relationship between the concentrations and peak areas was 10.04-100.4μg·mL-1(r=0.9998)for naringin,12.9-129μg·mL-1(r=0.9998)for hesperidin,17.44-174.4μg·mL-1(r=0.9997)for baicalin,the average recoveries of naringin,hesperidin and baicalin were 98.56%(RSD=1.30%),99.53%(RSD=0.92%)and 99.16%(RSD=1.32%)respectively.CONCLUSION The method is simple,accurate,and can be applied to the quantitative determination in this preparation.

Key concepts: Naringin, Hesperidin, Baicalin, Chromatography, Chemistry, High-performance liquid chromatography, Phosphoric acid, Paeoniflorin

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