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HPLC determination of hesperidin and baicalin in Xiao'er Runfeizhike oral liquid

Tan Sheng-jian

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Abstract

Objective:To develop an HPLC quantitative method for the determination of hesperidin and baicalin in Xiao'er Runfeizhike oral liquid.Methods:Column C18(150 mm×4.6 mm,5 μm),0.5% phosphoric acid(include 5% methanol)as mobile phase A,methanol as mobile phase B at flow rate of 1 mL·min-1.Gradient elution:0-45 min,A:90%→60%,B:10%→40%;and monitored at 284 nm.Results:The regress equation for hesperidin was Y=0.05488X+0.01433,r=0.9999 and the linear range was 2.690-33.63 μg·mL-1,The average recovery of hesperidin was 97.2%,RSD 1.1%.The regress equation for baicalin was Y=0.03710X+0.03698,r=0.9999 and the linear range was 7.969-99.61 μg·mL-1,The average recovery of baicalin was 101.3%,RSD 0.60%.Conclusion:This method is simple,time-saving and accurate.It can be used for routine analysis of hesperidin and baicalin in Xiao'er Runfeizhike Koufuye.

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What this paper is about

Objective:To develop an HPLC quantitative method for the determination of hesperidin and baicalin in Xiao'er Runfeizhike oral liquid.Methods:Column C18(150 mm×4.6 mm,5 μm),0.5% phosphoric acid(include 5% methanol)as mobile phase A,methanol as mobile phase B at flow rate of 1 mL·min-1.Gradient elution:0-45 min,A:90%→60%,B:10%→40%;and monitored at 284 nm.Results:The regress equation for hesperidin was Y=0.05488X+0.01433,r=0.9999 and the linear range was 2.690-33.63 μg·mL-1,The average recovery of hesperidin was 97.2%,RSD 1.1%.The regress equation for baicalin was Y=0.03710X+0.03698,r=0.9999 and the linear range was 7.969-99.61 μg·mL-1,The average recovery of baicalin was 101.3%,RSD 0.60%.Conclusion:This method is simple,time-saving and accurate.It can be used for routine analysis of hesperidin and baicalin in Xiao'er Runfeizhike Koufuye.

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Available abstract

Objective:To develop an HPLC quantitative method for the determination of hesperidin and baicalin in Xiao'er Runfeizhike oral liquid.Methods:Column C18(150 mm×4.6 mm,5 μm),0.5% phosphoric acid(include 5% methanol)as mobile phase A,methanol as mobile phase B at flow rate of 1 mL·min-1.Gradient elution:0-45 min,A:90%→60%,B:10%→40%;and monitored at 284 nm.Results:The regress equation for hesperidin was Y=0.05488X+0.01433,r=0.9999 and the linear range was 2.690-33.63 μg·mL-1,The average recovery of hesperidin was 97.2%,RSD 1.1%.The regress equation for baicalin was Y=0.03710X+0.03698,r=0.9999 and the linear range was 7.969-99.61 μg·mL-1,The average recovery of baicalin was 101.3%,RSD 0.60%.Conclusion:This method is simple,time-saving and accurate.It can be used for routine analysis of hesperidin and baicalin in Xiao'er Runfeizhike Koufuye.

Key concepts: Hesperidin, Baicalin, Chemistry, Chromatography, High-performance liquid chromatography, Phosphoric acid, Methanol, Linear range

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