2007•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Chlorogenic Acid in Yinhuang Particle by HPLC

Kang Hong-ying

Abstract

Objective To establish the determination method for chlorogenic acid in Yinhuang Particle.Methods Determination of Chlorogenic Acid in Yinhuang Particle by HPLC with C18column and acetonitrile-0.4%phosphoric acid(10∶90)as the mobile phase.The detection wavelengh was 327nm.Results The linear range was 0.164～0.984 μg·ml-1(r=1,n=6).The average recovery was 100.0%,RSD= 0.8%(n=4).ConclusionThe method is simple and accurate.It can offer the feasible basis for revision of the standard.

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Key concepts: Chlorogenic acid, Phosphoric acid, High-performance liquid chromatography, Chromatography, Chemistry, Linear range, Acetonitrile, Particle (ecology)