Determination of Chlorogenic Acid in Yinhuang Preparation by HPLC

Objective To establish a HPLC method for the determination of Chlorogenic Acid in Yinhuang preparation-Yinhuang tablet. During the determination of Chlorogenic Acid in Yinhuang oral liquid, the method is been compared with the UV method, offer gist for a more exactitude standard to control its quality. Methods A Hypersil ODS C18 column (5 μm, 150 mm×4.6 mm) is used with a mobile phase of methanol-water-acetic acid glacial-triethylamine (15∶85∶1.5∶0.3), the velocity of flow is 1.0 mL/min, the detect wavelength is 327 nm. Results The average recovery of Chlorogenic Acid is 98.92%, RSD=1.45%. Conclusion The method was simple, sensitivity and stable, and can be used as the method of quality control for Yinhuang tablet. The result that detected by UV method is not the actually concentration of Chlorogenic Acid. HPLC can detect it exactly, and is easier, more rapidly, fit for the quality control of Yinhuang preparation.