2007•Lishizhen Medicine and Materia Medica ResearchRequires access
Determination of the Content of Chlorogenic Acid in Yinhuang Granules from Different Pharmaceutical Factories by RP-HPLC
Zhao Yu
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Abstract
Objective To establish an HPLC method and determine the content of chlorogenic acid in Yinhuang Granules from different pharmaceutical factories.Methods RP-HPLC was used to determine the content of chlorogenic acid in Yinhuang granules.The column was Kromasil C18.The mobile phase was nitrile-methanol-water(containing 0.6% triethylamine,5∶15∶80),the detection wavelength was 327 nm.The column temperature was room temperature.Results There was a good linear relationship between the concentration of chlorogenic acid and absorption area value in range of 6.23×10-3～100.50×10-3 mg·ml-1,r=0.999 8.The average recovery was 98.30%,RSD= 2.35%(n= 6).Conclusion The chromatographic method established can be used simply and accurately to determine the content of chlorogenic acid in Yinhuang granules and it has good repeatability.