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2007•Anhui Medical and Pharmaceutical JournalRequires access

Content determination of chlorogenic acid in Yinhuang granules by HPLC

Wenli Liu

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Abstract

Aim To establish a method for the determination of chlorogenic acid in Yinhuang granules.Methods HPLC method was used.The samples were separated by VP-ODS column(150 mm×4.6 mm,5 μm).The mobile phase is actonitrile-0.4% acetic acid(13∶87).The detection wavelength was at 327 nm,with flow rate at 1.0 ml·min-1.The column temperature was indoor temperature.Results The linear range of schisandrin was 0.88～13.2 μg(r=0.999 9,n=5).The average rescovery was 99.39%,and RSD was 0.53%.Conclusion This method is sensitive,quick,accurate and can be used to determine the contents of chlorogenic acid in Yinhuang granules.

About this research paper

What this paper is about

Aim To establish a method for the determination of chlorogenic acid in Yinhuang granules.Methods HPLC method was used.The samples were separated by VP-ODS column(150 mm×4.6 mm,5 μm).The mobile phase is actonitrile-0.4% acetic acid(13∶87).The detection wavelength was at 327 nm,with flow rate at 1.0 ml·min-1.The column temperature was indoor temperature.Results The linear range of schisandrin was 0.88～13.2 μg(r=0.999 9,n=5).The average rescovery was 99.39%,and RSD was 0.53%.Conclusion This method is sensitive,quick,accurate and can be used to determine the contents of chlorogenic acid in Yinhuang granules.

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Available abstract

Aim To establish a method for the determination of chlorogenic acid in Yinhuang granules.Methods HPLC method was used.The samples were separated by VP-ODS column(150 mm×4.6 mm,5 μm).The mobile phase is actonitrile-0.4% acetic acid(13∶87).The detection wavelength was at 327 nm,with flow rate at 1.0 ml·min-1.The column temperature was indoor temperature.Results The linear range of schisandrin was 0.88～13.2 μg(r=0.999 9,n=5).The average rescovery was 99.39%,and RSD was 0.53%.Conclusion This method is sensitive,quick,accurate and can be used to determine the contents of chlorogenic acid in Yinhuang granules.

Key concepts: Chlorogenic acid, Chromatography, High-performance liquid chromatography, Chemistry, Acetic acid, Linear range, Detection limit, Biochemistry

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