Screening of pectinase producing strains and optimizing of its fermentation condition

A strain producing pectinase highly was screened through plate method from rotten jujube. The strain was identified as a bacterial of G-. A L16 (45) design was used to optimize the liquid submerged fermentation condition. The results showed that the optimum conditions were as carbon source at 1 %, nitrogen source at 0.5 %,surfactant 0.03 %, intial pH 4.5 and temperature 40 ℃. The activity of pectinase produced by the strain under the optimum condition reached 10 758 U·mL-1.