Breeding of a High Pectinase-producing Strain for Degumming of Raime and Optimizing Pectinase-producing Condition

Sixty pectinase-producing strains were separated from the soil under the roots of raime.A high pectinase-producing strain C-9 was obtained by screening with the methods of transparent zone and shaking culture from the 60 pectinase-producing strains.The initial pH value of fermentation medium,fermentation temperature,fermentation time and inoculation amount were optimized.Under the optimum conditions of pH value 7.5,temperature 35℃,time 36 h,inoculation amount 5%,the activity of pectinase got to 305.62 U·mL-1,as twice as that before optimization.