ISSR analysis on the inter-species relationship in Primulas
Zeng Shuhua
Abstract
Zeng Shuhua
Abstract
Inter-species relationship of 10 species in Primula were studied by using of ISSR marker.Eleven primers were screened out from forty random primers,and a total of 86 DNA fragments were amplified ranging from 300-2?000?bp.The average number of DNA fragments produced by each primer was 7.28.Nei'similarity coefficients and genetic distances were caculated by using POPGENE software and the dendrogram were constructed by using UPGMA.The 10 Primula species were divided into three groups.玃.poissonii Franch,P.Wilsoni Dunn,P.bulleyana Forr.,P.beesiana Forr.val.beesiana were clustered into one group,which is in consistence with the morphological classification.The relationships of P.poissonii Franch.and P.wilsoni Dunn.were discussed.
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Inter-species relationship of 10 species in Primula were studied by using of ISSR marker.Eleven primers were screened out from forty random primers,and a total of 86 DNA fragments were amplified ranging from 300-2?000?bp.The average number of DNA fragments produced by each primer was 7.28.Nei'similarity coefficients and genetic distances were caculated by using POPGENE software and the dendrogram were constructed by using UPGMA.The 10 Primula species were divided into three groups.玃.poissonii Franch,P.Wilsoni Dunn,P.bulleyana Forr.,P.beesiana Forr.val.beesiana were clustered into one group,which is in consistence with the morphological classification.The relationships of P.poissonii Franch.and P.wilsoni Dunn.were discussed.
Key concepts: UPGMA, Dendrogram, Similarity (geometry), Primula, Biology, Primer (cosmetics), Genetic distance, Genetic relationship