Analysis of Genetic Relationship of Rubus in Yunnan by ISSR

Genetic relationship of thirty nine Rubus species in Yunnan was studied by using inter-simple sequence repeat(ISSR) markers.Twelve polymorphic primers were screened out from 100 primers and 166 DNA fragments ranging from 259 bp to 2 300 bp were amplified with these primers.The average number of DNA fragments produced by each primer was 13.83.The polymorphic rate was 95.78%.UPGMA cluster analysis and NTSYS-pc software were used to construct the dendrogram based on the genetic distances.The 39 species were clustered into five groups namely section Idaeobatus Focke,section Malachobatus Focke,section Dalibardastrum Yu et Lu,section Chamaehatus Foke and section Cylactis Focke.,which was basically identical with previous results made by morphological study.Genetic distances between materials varied from 0.452 8 to 0.941 1,which showed that the genetic diversity among 39 Rubus species in Yunnan was abundant based on ISSR molecular markers.In addition,UPGMA clustering analysis showed significantly different genetic relationships among 39 species,and different species in the same section have close relationship,species in the different section have complicated difference.